SAFRANINE FOR MICROSCOPY

It is mostly utilized for the identification of cartilage, mucin, and mast cell granules. The safranin stain works by binding to acidic proteoglycans in cartilage tissues with a high affinity forming a reddish-orange complex. The binding made cartilage tissues appear red when observed under the microscope.

SAFRANINE SOLUTION is a fundamental biological stain, a red-pink dye used in histology, cytology, and microbiology to color acidic cell components like nuclei, cell walls (in plants), cartilage, and mucin, often as a counterstain in techniques like Gram staining to highlight Gram-negative bacteria or in plant histology (with Fast Green) to differentiate lignified tissues. It binds to negatively charged molecules (like nucleic acids and proteoglycans) to make structures visible under a microscope, providing crucial contrast for identifying different cell types and tissues.