Polyethylene Glycol 6000
Range of Avg. Molecular Weight 5400-6600
PEGs are soluble in water and most organic solvents. The reason for for using PEGs with large molecular weight is that smaller PEGs can diffuse through the cell wall and are even taken up by plant cells and distributed through the plant, which may influence the results. larger than 6,000 (PEG 6000) are inert, non-ionic and cell impermeable. to simulate osmotic stress.
Color of Solution, 5g/50ml of Water Slightly | Hazy |
Viscosity @98.9˚C | 250-390 Cst |
pH, 5% Solution in Water | 4.5-7.5 |
Residue on ignition Max | 0.1 %wt |
Assay (Average Molecular Weight) | 5400-6600 g/mol |
Heavy metals Max | 5 ppm |
Ethylene Oxide & 1,4-Dioxane Max. | 10 |
The reason for for using PEGs with large molecular weight is that smaller PEGs can diffuse through the cell wall and are even taken up by plant cells and distributed through the plant, which may influence the results. larger than 6,000 (PEG 6000) are inert, non-ionic and cell impermeable. to simulate osmotic stress.
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The addition of PEG to a protein solution increases the chemical potential of the protein. Precipitation of the protein occurs when its chemical potential exceeds the level of a saturated solution.