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Anionic surfactants work best to expel soil, clay, and some slick stains. These surfactants work following ionization. At the point when added to water, the anionic surfactants ionize and have a negative charge. The negatively charged surfactants bind to decidedly charged particles like clay. Anionic surfactants are viable in removing particulate soils. Nonionic surfactants are particularly productive at removing sleek soils from manufactured fabrics yet they are not as proficient at removing particulate soils as anionic surfactants.

Crystal violet

Synthetic Names: Crystal violet; Gentian violet, Basic violet

Gentian violet is a color that is a blend of violet rosanilinis with antibacterial, antifungal, and anthelmintic properties.

Giemsa stains

It is particular for the phosphate gatherings of DNA and appends itself to locales of DNA where there are high measures of adenine-thyminebonding. Giemsa stains is utilized as a part of Giemsa banding, regularly called G-banding, to recolor chromosomes and frequently used to make a karyogram (chromosome outline). It can recognize chromosomal distortions, for example, translocations and adjustments.

It recolors the trophozoite Trichomonas vaginalis, which presents with greenish release and motile cells on wet prep.